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KMID : 0948320050050020159
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Konyang Medical Journal
2005 Volume.5 No. 2 p.159 ~ p.167
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Microsatellite Alterations of Chromosome 2, 5, 8, 17 in Intrahepatic Cholangiocarcinoma
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Choi In-Seok
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Abstract
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Background.: Intrahepatic cholangiocarcinoma (ICC) is the second most frequent malignant tumor in the liver after hepatocellular carcinoma (HCC), but the study on molecular, tumor biological and clinical approaches related to the prognostic factors have been rarely reported. Compared to HCC, ICC has some different tumor biological characteristics and has the propensity for late diagnosis. Recently much efforts for elucidation of the early cholangiocarcinogenesis have been made, and for this purpose it is crucial to investigate the genetic abnormalities. We have proposed to evaluate the general pattern of cholangiocarcinoma including clinicopathological findings, the molecular mechanism of cholangiocarcinogenesis, the significance of microsatellite alterations associated with the genesis of cholangiocarcinoma, and the correlation with clinicopathological prognostic factors.
Materials and Method. :We have evaluated microsatellite alterations study of nine markers (BAT26, D2S123, D5S346, D17S250, D8S254, D8S261, D8S262, D17S796, TP53) from chromosome 2, 5, 8, 17 in 25 cases of surgically resected ICC, 10 cases of HCC and 10 cases metastatic adenocarcinoma. Additionally p53 gene mutation and p53 protein expression were studied.
Results.:Microsatellite instability (MSI) was detected in 6 cases (24%) overall, with frequencies showing BAT26 (3 cases), D2S123 (3 cases), D17S250 (2 cases). Loss of heterozigosity(LOH) of D8S254, D8S261, D8S262, D17S250, TP53, D17S796 were detected in 6/19 (31.5%), 4/22 (18.1%), 1/23 (4.3%), 3/22 (13.6%), 5/21 (23.8%), 4/17 (23.5%), respectively. 4 cases having 2 or more LOH were poorly differentiated and showed lymphatic emboli or vascular invasion. In HCC, MSI was detected 1/10 (10%), LOH of D8S262, D17S796, TP53 were 3/9 (33.3%), 3/9 (33.3%), 3/8 (37.5%), respectively. p53 gene mutation was detected in 12 (48%) cases and p53 overexpression was measured in 13 (53%) cases.
Conclusion. :As a result, we concluded that alterations in microsatellite of various chromosom es may contribute to the cholangiocarcinogenesis and tumor progression.
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KEYWORD
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Intrahepatic cholangiocarcinoma, Microsatellite
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